Diet induced obesity in rats pdf files

In the present study of the antiobesity effect of capsaicin, rats 5week old received capsaicin 10 mgkg along with a highfat diet hfd. Obesity epidemic warrants scientific attention in the u. A controlled highfat diet induces an obese syndrome in rats. Therefore, the absence of diet induced thermogenesis in ko mice in the first days following a highfat diet switch may largely explain their profound susceptibility to diet induced obesity. Fatty diet is a significant factor causing obesity. Diet rich in fat induced obesity not only in human but also in animals. Custom diet protocols can use any diet manufactured according. Dietinduced obesity in rats leads to a decrease in sperm motility article pdf available in reproductive biology and endocrinology 91. Effect of consumption of a highfat hf diet on body weight, adiposity, and food intake in lowfat lf and hffed animals after 8 or 12 wk on respective diets a.

Here, we demonstrate that supplementation with low doses 14 and 140 mg per kg per rat of a complete cocoa extract induces metabolic benefits in a diet induced obesity dio model of wistar rats. Jul 15, 2015 in the past decades, obesity and associated metabolic complications have reached epidemic proportions. Rodent model for the study of obesityrelated disorders 387rev assoc med bras 2012. Control males are housed in the same location, also at reduced density, and are fed nih31m diet control males can also be generated using a low fat purified diet upon request. This is likely to result from the additive effects of obesity and pregnancy on insulin sensitivity.

Effect of carnitine and herbal mixture extract on obesity induced by high fat diet in rats. Thus, the aim of the present study was to investigate the effect of highfat dietinduced obesity on male reproductive parameters. Intraventricular injection of lkb1 inhibits the formation of. The effect of diet induced obesity on ischaemic stroke cohort 2 figure 15. Inquire about customized diet induced obese dio models using any of taconics commercial animal models. The sd spraguedawley rat model of dio diet induced obesity is reported to exhibit a clear segregation into susceptible and resistant subpopulations shortly after transfer to a he high energy diet. Measuring conditions include nutritional status of the subject, physical activity and duration of.

Dietinduced obesity in rats leads to a decrease in sperm. Longterm characterization of the dietinduced obese and diet. However, little is known about the contribution of 3,5,6,7,8,3. Pdf dietinduced obesity in rats leads to a decrease in. Characterization of the satellite glial cell sgc in the. Obesity is rapidly becoming a worldwide epidemic that affects children and adults.

In this study, it was purposed to examine histological effects of fatty diet induced obesity on liver from female obese rats. Protective effects of tamarillo cyphomandra betacea. The average food and water intake of each rat are presented in table 1. Body weights of male rats fed the highfat diet hf in expt 1 n 16, expt 2 n 8, expt 3 n 16 and female rats in expt 4 n 8 as a percentage of the body weight of rats fed the lowfat diet lf over 10 wk. Methods female wistar rats were fed either a standard chow 3% fat, 7% sugar wtwt or a palatable obesogenic diet 11% fat, 43% sugar wtwt for 8 weeks before mating and throughout pregnancy and lactation. Jun 25, 2019 obesity is a risk factor for chronic kidney disease ckd. A total 40 rats were randomly assigned into two groups, normal 10 rats and obese 30 rats. Rats were fed on either normal chow or high fat diet for 10 weeks for obesity induction phase and subsequently received c.

Current study comprises usage of a dietinduced obesity model by proposing high fat diet on adult female rats and examination of the livers of these obese rats under light microscope. Using dietinduced obesity to understand a metabolic subtype. Controlled highfat diet induces an obese syndrome in rats. Diet induced obesity in rats is associated with glucose intolerance during pregnancy but not in the nonpregnant state. Modeling dietinduced obesity with obesityprone rats. Established dietinduced obesity in female rats leads to. Characterisation of ischaemic stroke in dietinduced obese.

In general, a highfat hf diet induces the development of metabolic. This obese rat model is an attractive model to study further the physiologic and molecular abnormalities in gdm. Herzog yz y human performance laboratory, university of calgary, ab, canada z mccaig institute for bone and joint health, university of calgary, ab, canada. Blood glucose levels of control and high fat diet hfd fed rats cohorts 14. Many previous studies performed on mice and rats suggested that obesity can be induced by high fat diet 21. In this present study, the high fat diet consists of 40% of fat of total energy. Male sprague dawley rats were fed a westernstyle 21% fat by weight. According to another survey, the global prevalence of obesity has. Effect of carnitine and herbal mixture extract on obesity. However, a direct comparison between wistar and spraguedawley sd rat as models of highfat hf dietinduced obesity has not been adequately evaluated so far. Treating obese and diabetic rats with enalapril or a menhaden oil enriched diet generally improved peripheral neuropathy endpoints. After administration, the rats were fed a highfat diet hfd for 9 weeks to induce obesity.

Find, read and cite all the research you need on researchgate. When fed ad libitum with a highfat diet hfd, obesity, hyperinsulinemia, hyperglycemia and hypertension establish in these mice. Longterm hfd leads to damage, inflammation and fibrosis in the kidneys of aging rats. Effects of different diets used in dietinduced obesity models on. A blood sample was taken from the tail vein before the start of the study at 70 days of age for the measurement of glucose, insulin, and leptin. The effect of diet induced obesity on ischaemic stroke cohort 1 figure 14. Lkb1 also improved hfdinduced hepatic fatty degeneration. However, a direct comparison between wistar and spraguedawley sd rat as models of highfat hf diet induced obesity has not been adequately evaluated so far. Comparison of dietary control and atorvastatin on high fat. Butyrate has been reported to attenuate dietinduced obesity and insulin resistance. Highfat dietinduced obesity causes an inflammatory. The aim of this research was to confirm whether a combination of quercetin and resveratrol cqr could restore the gut microbiota dysbiosis induced by a highfat diet hfd. In addition, after junkfood diet exposure, those rats that developed obesity also showed increased willingness to gain access to a sucrose cue. Diet induced and genetic models of obesity have been.

Table 1 a different types of diet used and dietinduced body and metabolic alterations dietinduced obesity. We applied a comparative functional genomics approach to evaluate whether diet. Rats were fed a high fat diet and treated with a low dose of streptozotocin to create models of diet induced obesity or type 2 diabetes. Antiobesity and hypolipidemic activity of moringa oleifera.

In the past decades, obesity and associated metabolic complications have reached epidemic proportions. In a first experiment, male wistar rats were fed a highfat diet hfd. Treatment with atorvastatin ato or dietary control has been demonstrated to benefit patients with nonalcoholic fatty liver disease nafld and hyperlipidemia. Individual differences in cueinduced motivation and. Here, we demonstrate that supplementation with low doses 14 and 140 mg per kg per rat of a complete cocoa extract induces metabolic benefits in a dietinduced obesity dio model of wistar rats.

May 01, 2017 the fact that rats of the cafeteria diet group initially ingested a lower quantity of food feed and cafeteria diet may be related to a compensatory mechanism of the organism in relation to the high energy level of the cafeteria diet. Some studies have shown a relationship between obesity and infertility, but until now it remains controversial. In this study, wistar rats were divided into three groups. This study was conducted to determine the mechanisms implicated in the beneficial effects of apple polyphenols aps against diet. The fact that rats of the cafeteria diet group initially ingested a lower quantity of food feed and cafeteria diet may be related to a compensatory mechanism of the organism in relation to the high energy level of the cafeteria diet. Highfat diet increases pain behaviors in rats with or.

It is known that obesity is associated with many chronic disorders such as nonalcoholic fatty liver disease nafld. The dietinduced obesity model dio model is an animal model used to study obesity using animals that have obesity caused by being fed highfat or highdensity diets. Impact of chromium histidinate on high fat diet induced. Hypercaloric diets are tightly related to the obesity etiology and also cause alterations in gut microbiota functionality. The role of o1602 and o1918 in attenuating obesity and obesityrelated pathologies is unknown. Mar 11, 2011 obesity is rapidly becoming a worldwide epidemic that affects children and adults. In comparison with salinetreated rats, body weight of those in the capsaicintreated. Over the last two decades, we and others have employed an outbred rat model to study the development and persistence of obesity, as well as. Obesity was induced in rats for 70 day by feeding the high fat diet. Rodents with dietinduced obesity dio are widely used as models of obesity. Butyrate may represent a mechanism for the obesityattenuating effects of prebiotics butyrate, a shortchain fatty acid produced by gut microbe fermentation of dietary fiber, was increased in the cecum and colon of these prebiotictreated mice. Role of butyrate in restoring satiety signaling in rodent. Daily energy expenditure consists of three components.

While the exact mechanisms remain unclear, inflammation may be a consequence of obesity that directly impacts the kidneys. Pdf dietinduced obesity in rats leads to a decrease in sperm. Ontogeny of dietinduced obesity in selectivelybred rats. In four experiments, longevans rats ate ad libitum a. Therefore, we aimed to determine the role that either compound had on body weight and body composition, renal and hepatic function in dietinduced obesity. Rat models of dietinduced obesity and high fatlow dose. Over the past two decades, some confusion has emerged in the terminology used in describing outbred models.

Obesity and its comorbidities are currently considered an epidemic, and the involved pathophysiology is well studied. We examined whether reversal of high fat diet, stimulating weight loss, compared to two treatments previously shown to have beneficial effects, could improve glucose utilization and peripheral neuropathy in animal models of obesity and type 2 diabetes. Diet induced obesity in zebrafish shares common pathophysiological pathways with mammalian obesity. Since rats and mice show a similar relationship, they are considered an appropriate model. Request pdf on sep 1, 2001, mr ricci and others published ontogeny of dietinduced obesity in selectivelybred rats. Measuring conditions include nutritional status of the subject, physical activity and duration of the observation. We found that rats susceptible to dietinduced obesity displayed heightened conditioned approach prior to the development of obesity. A combination of quercetin and resveratrol reduces obesity.

To investigate the potential consequences of hfd induced longterm obesity on kidney damage, inflammation and fibrosis, we first performed comprehensive analyses of passtained renal sections fig. Proteomic analysis for antiobesity potential of capsaicin. While body weight is typically used in diet induced models of obesity as the measure of obesity in rats, it may not be the best measure. Diet induced obese dio mouse models c57bl6ntac male mice. Obesity is a risk factor for chronic kidney disease ckd. Male spraguedawley rats were fed a highfat diet 40% digestible energy from lipids. Wistar rats are also used in studies where obesity is diet induced, and the results have shown that the body weight is increased.

Diet and antibiotics are known to play crucial roles in changes in the microbiota ecosystem and the disruption of its balance. This does not appear to be the case for rats sourced in the u. Lkb1 also improved hfd induced hepatic fatty degeneration. Rats on a cafeteriastyle caf diet 10 had free access to a semisolid diet containing 33% ground commercial rat chow, 33% full fat sweetened condensed milk nestle, 7% sucrose, and 27% water. After 10 weeks, cocoa extractsupplemented animals exhibited significantly lower body weight gain and food efficiency, with no differences in energy. High fat diet induced obesity impairs skeletal muscle. Values are mean sem the proportional increase of body weight of hf rats relative to lf rats followed a comparable trajectory for cohorts studied at different times of the. Proteomic analysis for antiobesity potential of capsaicin on. Diet induced obese dio mouse models c57bl6ntac male. Jun 11, 2015 after receiving their respective diet for 10 weeks, the obese induced rats gained significantly higher body weight and bmi than the negative control rats, thus verified to be obese.

Low doses of cocoa extract supplementation ameliorate diet. Rats fed a chow fat diet were used as normal controls. Aug 24, 2018 recent data demonstrate that hypothalamic activation of the unfolded protein response upr plays a primary pathogenic role in obesity. For the study of these pathologies, a number of animal models have been developed. Wet weight and cellularity of dissected adipose depots of adult rats fed a highfat diet for 5 mo gonadal bdy wt, retroperitmeal inguinal g total lipid, cell size, pg no. We employed a rat model of nafld to examine the therapeutic efficacy of. As mentioned in table 3, it has been observed that rats feed on highfat diet consecutively for 21 days resulted in a marked increase in the level of lipids, characterized by elevated levels of total cholesterol, triglycerides, ldl, vldl, and reduced levels of hdl when compared to normal control, that is, rats receiving the normal feed.

Vagal nerves are perfectly positioned to convey peripheral metabolic status to the brain. In the kidneys of 75weekold rats with continuous hfd consumption, we detected a significant increase in. Development of a new dietinduced obesity dio model using. Highfat dietinduced obesity in animal models niloofar hariri and louise thibault school of dietetics and human nutrition, mcgill university, quebec, canada epidemiological studies have shown a positive relationship between dietary fat intake and obesity. Typically mice, rats, dogs, or nonhuman primates are used in these models. Original article berberine protected rats against adiposity. Oct 16, 2009 a total 40 rats were randomly assigned into two groups, normal 10 rats and obese 30 rats. Epigenetic dysregulation of the dopamine system in diet.

Highfat dietinduced metabolic syndrome and oxidative stress in. Highfat diet consumption for 8 weeks affected body weight in longevans but not spraguedawley rats. Intraventricular injection of lkb1 inhibits the formation. However, little is known on whether combination of dietary control and ato treatment could enhance the therapeutic effect. Propensity to highfat dietinduced obesity in rats is. Thus, the aim of the present study was to investigate the effect of highfat diet induced obesity on male reproductive parameters. The effect of diet induced obesity on ischaemic stroke cohort 3. Normal group, 10 rats fed on normal diet during the entire study. Marques c 1, meireles m2, norberto s2, leite j 2, freitas j 3, pestana d1, faria a4, calhau c 1. It is well recognized that capsaicin increases thermogenesis through enhancement of catecholamine secretion from the adrenal medulla. Highfat diet induced obesity in animal models niloofar hariri and louise thibault school of dietetics and human nutrition, mcgill university, quebec, canada epidemiological studies have shown a positive relationship between dietary fat intake and obesity. The diet induced obesity model dio model is an animal model used to study obesity using animals that have obesity caused by being fed highfat or highdensity diets.

The composition and preparation techniques of yogurt are provided in a supplementary file. Wistar rats are also used in studies where obesity is dietinduced, and the results have shown that the body weight is increased. As mentioned in table 3, it has been observed that rats feed on highfat diet consecutively for 21 days resulted in a marked increase in the level of lipids, characterized by elevated levels of total cholesterol, triglycerides, ldl, vldl, and reduced levels of hdl when compared to normal control, that is, rats receiving the normal. The rats were included in four groups after induction of obesity. The effects of diet on body weight are inconsistent such that some studies suggest that a highfat and a highsugar diet will result in weight gain while others suggest that only a highfat diet will lead to. To charles river north america in 1975 from charles river uk.

In conclusion, our results provide in vivo genetic support for a role for adipose tissue gatm and creatine metabolism in energy expenditure and the defense. Pdf dietinduced obesity in zebrafish shares common. Berberine protected rats against adiposity 151 int j clin exp med 2016. Dietinduced adipocyte number increase in adult rats. We developed a rat model of dietinduced obesity that will be a powerful tool for assessment of this issue. Effects from dietinduced gut microbiota dysbiosis and.

Lkb1 delivery decreased body weight, energy intake, fat mass, and serum lipid levels. It is intended to mimic the most common cause of obesity in humans. Therefore, the absence of dietinduced thermogenesis in ko mice in the first days following a highfat diet switch may largely explain their profound susceptibility to dietinduced obesity. In this study, we used the paradigm of diet induced obesity first described by woods et al. Using dietinduced obesity to understand a metabolic. However, its metabolic role in the hypothalamus remains unknown. This study aims to investigate the protective effect of cyphomandra betacea in adult male spraguedawley rats fed with high fat diet. Apr 01, 2010 changes in expression of skeletal muscle proteins between obesity prone and obesity resistant rats induced by a highfat diet. This particular colony was selected because of a low incidence of hydronephrosis. One such polygenetic model is the dietinduced obese dio and dietresistant dr outbred rat models levin et al. Individual differences in cueinduced motivation and striatal.

The data showed that hfdinduced obesity in lev rats was. Protective effects of tamarillo cyphomandra betacea extract. Dietinduced obesity in rats is associated with glucose intolerance during pregnancy but not in the nonpregnant state. Control c rats had free access to a standard nonpurified diet containing 51% carbohydrate, 4% fat, and 21% protein. Figure 1 demonstrates that diet induced obese rats figure 1a, area under the curve auc. Using diet induced obesity to understand a metabolic subtype of osteoarthritis in rats k. We aimed to investigate the potential efficacy of hmf on preventing obesity and hyperlipidemia in rats fed a highfat diet hfd and its underlying mechanisms.

While body weight is typically used in dietinduced models of obesity as the measure of obesity in rats, it may not be the best measure. Individual differences in cueinduced motivation and striatal systems in rats susceptible to dietinduced obesity mike jf robinson1,2, paul r burghardt3,4, christa m patterson5, cameron w nobile6, huda akil3, stanley j watson3, kent c berridge1 and carrie r ferrario,6 1department of psychology, university of michigan, ann arbor, mi, usa. The aim of this study was to examine the inflammatory status of the kidneys and potential ongoing renal damage, i. Using dietinduced obesity to understand a metabolic subtype of osteoarthritis in rats k.